Coding

Part:BBa_K1334016:Design

Designed by: Qiushuang Wu   Group: iGEM14_WHU-China   (2014-09-27)


formate dehydrogenase(FDH) from Escherichia.coli DH5a


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1260
    Illegal BglII site found at 1499
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 955
    Illegal AgeI site found at 1774
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence of FDH from E.coli contains a TGA,but in fact,it can be translated into Se-methylselenocysteine


Source

We PCR from the genome of E.coli,DH5a strain.

References